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Image Search Results
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems,
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primers for reverse transcription–quantitative PCR
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems,
Techniques:
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Characterization of tubular cell phenotypes and injury in rodents with 2,8-DHA nephropathy. (A) The injury marker Lcn2 (lipocalin-2) was progressively upregulated during the murine nephropathy time course on the mRNA level. (A’) Crystals (stars) were adherent and extratubulated in (A’’) LCN2− as well as (A’’’) LCN2+ tubules (pictures from mouse). (B) Tubules with extratubulating crystals were KIM-1+ (arrowheads). Cells that migrated on the crystal (stars), however, were KIM-1− in most (first two examples, arrows) but not all cases (third example, arrow, images from rats). (C) Immunofluorescence double staining showed that tubules with crystals were either LCN2+ or KIM-1+. (D) At sites of extratubulation, we only observed proliferation of tubular epithelial cells visualized by mitotic figures in PAS sections or by PCNA immunohistochemistry directly next to the 2,8-DHA crystal, but never in cells that overgrew the crystal (images from rats). Immunohistochemistry quantification and representative images showed increased expression of (E) TNFR1, (F) TNFR2, and (G) CD44 during the time course of the 2,8-DHA nephropathy in mice. Scale bars, 50 or 25 µm. *P<0.05, †P<0.01, ‡P<0.001, #P<0.0001 versus day 1. d, day.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems,
Techniques: Marker, Immunofluorescence, Double Staining, Immunohistochemistry, Expressing
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Deletion of Tnfr1 but not Tnfr2 ameliorated 2,8-DHA nephropathy. (A) Experimental design. Compared with wild-type mice, Tnfr1−/− but not Tnfr2−/− had (B) significantly reduced serum urea concentration and (C) slightly lowered serum creatinine. (D) Lcn2 mRNA expression tended to be reduced in both mouse lines. (E) CD44 expression, (F) the number of intrarenal crystals in kidney sections stained with HE and viewed by polarized light, as well as (G) tubulointerstitial injury scores of PAS sections were significantly reduced in Tnfr1−/−. Reduced inflammation was detected by (H) significantly decreased Il6 mRNA expression and (I) significantly decreased infiltration of F4/80+ cells. Representative TNFR1 immunofluorescence images in kidneys from (J) healthy individuals and from (K) patients with APRT deficiency, as well as (L) immunohistochemistry of a kidney from a mouse fed a 0.2% adenine diet. In healthy human kidneys, TNFR1 was only expressed by a few tubular epithelial cells in a luminal, cytoplasmic, or granular pattern (arrowheads). In human and murine kidneys with 2,8-DHA crystal nephropathy, TNFR1 was upregulated and expressed by dilated tubules (arrowheads) and tubules with extratubulating crystals (stars). Scale bars, 25 or 50 µm, respectively. wt, wild-type. *P<0.05 versus wt.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems,
Techniques: Concentration Assay, Expressing, Staining, Immunofluorescence, Immunohistochemistry
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Neither deletion of Cd44 nor of Ahsg was protective against 2,8-DHA nephropathy. (A) Experimental design of studies in Cd44 mice. No differences between Cd44−/− and Cd44+/− mice after 7 days of 0.2% adenine diet were detected for the kidney function parameters (B) creatinine clearance, (C) serum creatinine, (D) serum urea, or (E) systolic BP. Furthermore, there were no differences in the amount of (F) intrarenal crystals in HE-stained kidney sections, (G) mRNA expression of Lcn2, or (H) tubulointerstitial injury scored in PAS sections. (I) Expression of F4/80 was significantly reduced in Cd44−/− mice, whereas there was (J) no difference in collagen III accumulation. (K) Experimental design in Ahsg mice. No differences could be detected between Ahsg−/− and wild-type (Ahsg+/+) mice in terms of (L) creatinine clearance, (M) serum creatinine, (N) serum urea, (O) systolic BP, (P) amount of intrarenal 2,8-DHA crystals, (Q) Lcn2 expression, (R) extent of tubulointerstitial injury, (S) F4/80 expression, and (T) collagen type III expression after 14 days of adenine-enriched diet. Col. collagen; Crea., creatinine; Ctrl, control (i.e., wild-type or hemizygous, respectively). *P<0.05 versus Ctrl.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems,
Techniques: Staining, Expressing
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam,
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent,
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310) R&D Systems, MAB1757 (US) Lipocalin-2 (NGAL) (for double staining) Rabbit-anti-NGAL (polyclonal) Thermo Fisher Scientific, PA5–79590 (US) Cytokeratin (Pan CK) Mouse-anti-pan Cytokeratin (clone Lu5) Abcam, ab17155 (UK) Proliferation (Ki-67, MIB1) Mouse-anti-human (clone KI-67P) Dianova,
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad,
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primary antibodies for immunohistochemistry or immunofluorescence staining
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310)
Techniques: Immunohistochemistry, Immunofluorescence, Double Staining
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Primers for reverse transcription–quantitative PCR
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310)
Techniques:
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Characterization of tubular cell phenotypes and injury in rodents with 2,8-DHA nephropathy. (A) The injury marker Lcn2 (lipocalin-2) was progressively upregulated during the murine nephropathy time course on the mRNA level. (A’) Crystals (stars) were adherent and extratubulated in (A’’) LCN2− as well as (A’’’) LCN2+ tubules (pictures from mouse). (B) Tubules with extratubulating crystals were KIM-1+ (arrowheads). Cells that migrated on the crystal (stars), however, were KIM-1− in most (first two examples, arrows) but not all cases (third example, arrow, images from rats). (C) Immunofluorescence double staining showed that tubules with crystals were either LCN2+ or KIM-1+. (D) At sites of extratubulation, we only observed proliferation of tubular epithelial cells visualized by mitotic figures in PAS sections or by PCNA immunohistochemistry directly next to the 2,8-DHA crystal, but never in cells that overgrew the crystal (images from rats). Immunohistochemistry quantification and representative images showed increased expression of (E) TNFR1, (F) TNFR2, and (G) CD44 during the time course of the 2,8-DHA nephropathy in mice. Scale bars, 50 or 25 µm. *P<0.05, †P<0.01, ‡P<0.001, #P<0.0001 versus day 1. d, day.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310)
Techniques: Marker, Immunofluorescence, Double Staining, Immunohistochemistry, Expressing
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Deletion of Tnfr1 but not Tnfr2 ameliorated 2,8-DHA nephropathy. (A) Experimental design. Compared with wild-type mice, Tnfr1−/− but not Tnfr2−/− had (B) significantly reduced serum urea concentration and (C) slightly lowered serum creatinine. (D) Lcn2 mRNA expression tended to be reduced in both mouse lines. (E) CD44 expression, (F) the number of intrarenal crystals in kidney sections stained with HE and viewed by polarized light, as well as (G) tubulointerstitial injury scores of PAS sections were significantly reduced in Tnfr1−/−. Reduced inflammation was detected by (H) significantly decreased Il6 mRNA expression and (I) significantly decreased infiltration of F4/80+ cells. Representative TNFR1 immunofluorescence images in kidneys from (J) healthy individuals and from (K) patients with APRT deficiency, as well as (L) immunohistochemistry of a kidney from a mouse fed a 0.2% adenine diet. In healthy human kidneys, TNFR1 was only expressed by a few tubular epithelial cells in a luminal, cytoplasmic, or granular pattern (arrowheads). In human and murine kidneys with 2,8-DHA crystal nephropathy, TNFR1 was upregulated and expressed by dilated tubules (arrowheads) and tubules with extratubulating crystals (stars). Scale bars, 25 or 50 µm, respectively. wt, wild-type. *P<0.05 versus wt.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310)
Techniques: Concentration Assay, Expressing, Staining, Immunofluorescence, Immunohistochemistry
Journal: Journal of the American Society of Nephrology : JASN
Article Title: Cellular and Molecular Mechanisms of Kidney Injury in 2,8-Dihydroxyadenine Nephropathy
doi: 10.1681/ASN.2019080827
Figure Lengend Snippet: Neither deletion of Cd44 nor of Ahsg was protective against 2,8-DHA nephropathy. (A) Experimental design of studies in Cd44 mice. No differences between Cd44−/− and Cd44+/− mice after 7 days of 0.2% adenine diet were detected for the kidney function parameters (B) creatinine clearance, (C) serum creatinine, (D) serum urea, or (E) systolic BP. Furthermore, there were no differences in the amount of (F) intrarenal crystals in HE-stained kidney sections, (G) mRNA expression of Lcn2, or (H) tubulointerstitial injury scored in PAS sections. (I) Expression of F4/80 was significantly reduced in Cd44−/− mice, whereas there was (J) no difference in collagen III accumulation. (K) Experimental design in Ahsg mice. No differences could be detected between Ahsg−/− and wild-type (Ahsg+/+) mice in terms of (L) creatinine clearance, (M) serum creatinine, (N) serum urea, (O) systolic BP, (P) amount of intrarenal 2,8-DHA crystals, (Q) Lcn2 expression, (R) extent of tubulointerstitial injury, (S) F4/80 expression, and (T) collagen type III expression after 14 days of adenine-enriched diet. Col. collagen; Crea., creatinine; Ctrl, control (i.e., wild-type or hemizygous, respectively). *P<0.05 versus Ctrl.
Article Snippet: Additional histology samples were stained with van Kossa or silver stain, or immunohistochemistry against keratin, CD68, Ki-67, or TNFR1. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Protein Antibody Company α -SMA Mouse-anti-human SMA (clone 1A4) Dako/Agilent, M085101–2 (US) Aquaporin-2 Rabbit-anti-AQ2 (polyclonal) Abcam, ab15116 (UK) CD3 Rat-anti-human CD3 (cloneCD3–12) Bio-Rad, MCA1477 (GER) CD11b Rat-anti-mouse CD11b (clone M1/70) PE-labeled eBioscience/Thermo Fisher Scientific, 12–0112–82 (US) CD11c Hamster-anti-mouse CD11c (clone N418) APC-labeled eBioscience/Thermo Fisher Scientific, 17–0114–82 (US) CD13 Rabbit-anti-CD13 (clone EPR4058) Abcam, ab108310 (UK) CD44 Rat-anti-mouse CD44 (clone IM7) BD Pharmingen, 553131 (US) CD68 Mouse-anti-rat CD68 (clone ED1) Bio-Rad, MCA341R (GER) CD68 Mouse-anti-human CD68 (clone KP1) Dako/Agilent, M081401 (US) Collagen III Goat-anti-type III collagen (antiserum) Southern Biotech, 1330–01 (US) Murine monocytes/macrophages (ER-HR3) Rat-anti-mouse monocyte antigen (clone ER-HR3) BMA Biomedicals, T-2012 (CHE) F4/80 Rat-anti-mouse F4/80 (clone CI:A3–1) Bio-Rad, MCA497G (GER) Fibronectin Rabbit-anti-rat fibronectin (polyclonal) Millipore/Merck AB1954 (GER) KIM-1 Goat-anti-mouse TIM-1/KIM-1/HAVCR (polyclonal) R&D Systems, AF1817 (US) Lipocalin-2 (NGAL) Rat-anti-human lipocalin-2/NGAL (clone 220310)
Techniques: Staining, Expressing